| |
|
|
| Registros recuperados : 10 | |
| 4. |  | CALLEROS,L.; BARCELLOS M.; BETANCOR, L.; DELPIAZZO, R.; FRAGA, M.; IRAOLA, G.; MORSELLA, C.; PAOLICCH, F.; PÉREZ ,R. Detección e identificación rápida de Campylobacter fetus en el ganado bovino mediante métodos moleculare. [Resumen]. En: CONGRESO ASOCIACIÓN URUGUAYA DE PRODUCCIÓN ANIMAL (6º, Marzo, 2018, Tacuarembó, Uruguay). Tacuarembó: AUPA, 2018. p. 146.| Biblioteca(s): INIA La Estanzuela. |
|    |
| 5. | | COSTA, D.; ARÁOZ, V.; BARCELLOS, M.; CAFFARENA, D.; FRAGA, M.; GIANNITTI, F.; MONESIGLIO, M.C.; PÉREZ , R.; SILVEIRA, C.S.; CALLEROS, L. Complete genome sequence of campylobacter fetus isolated from a sheep. Microbiology Resource Announcements, 5 November 2020, Volume 9, Issue 45, Article number e01008-20. Open Access. Doi: https://doi.org/10.1128/MRA.01008-20 Article history: Received 28 August 2020/ Accepted 20 October 2020/ Published 5 November 2020.| Biblioteca(s): INIA La Estanzuela. |
| |
| 6. | | DELPIAZZO, R.; BARCELLOS, M.; BARROS, S.; BENTANCOR, L.; FRAGA, M.; GIL, J.; IRAOLA, G.; MORSELLA, C.; PAOLICCHI, F.; PÉREZ, R.; RIET-CORREA, F.; SANGUINETTI, M.; SILVA, A.; SILVEIRA, C.S.; CALLEROS, L. Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence. Veterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163 Article history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020.
Corresponding author: laurabet@higiene.edu.uy| Biblioteca(s): INIA Treinta y Tres. |
| |
| 7. | | DURAN, H.; HOYOS, C.; BERTI, D.; MIRANDA, W.; PÉREZ, R.; VILLAMONTE, L. (Coord.). Predios pilotos lecheros. La Estanzuela (Uruguay): INIA, 1991. 15p Convenio : INIA, ANPL, CONAPROLE, Con la cooperación del Gobierno de la República Federal de Alemania a través de su agencia GTZ.| Biblioteca(s): INIA La Estanzuela. |
| |
| 9. | | NAVAJAS, E.; VELAZCO, J.I.; SOTELO, J.; NOYA, H.; CASAL, A.; GIANOLA, D.; PERAZA, P.; BIELLI, A.; ITHURRALDE, J.; GENOVESE, P.; PÉREZ, R.; CANTALAPIEDRA, G.; AGUILAR, I.; LEMA, O.M.; RAVAGNOLO, O.; DEVINCENZI, T.; CARRACELAS, B.; DE SOUZA, G.; CIAPPESONI, G.; BRITO, G.; LUZARDO, S. Plataforma de Fenotipado intensivo - Kiyú. Anuario Hereford (Montevideo), 2020, p. 210-213.| Biblioteca(s): INIA La Estanzuela; INIA Tacuarembó. |
|  |
| 10. | | PÉREZ, R.; MARANDINO, A.; TOMÁS, G.; PANZERA, Y.; HERNÁNDEZ, M.; HERNÁNDEZ, D.; MILANO, A.; TECHERA, C.; GRECCO, S. Incorporación de técnicas moleculares y bioinformáticas en avicultura para la investigación epidemiológica y el diseño de estrategias de control y prevención de
Gumboro y Bronquitis Infecciosa. Montevideo (UY): INIA, 2019. 35 p. (Serie FPTA-INIA; 74). Proyecto FPTA-319: Incorporación de técnicas moleculares y bioinformáticas en avicultura para la investigación epidemiológica y el diseño de estrategias de control y prevención de Gumboro y Bronquitis Infecciosa. Periodo de ejecución:...| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
| |
| Registros recuperados : 10 | |
|
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha actual : |
08/06/2021 |
|
Actualizado : |
02/09/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
GASTAL, G.D.A.; SCARLET D.; MELCHERT M.; ERTL R.; AURICH, C. |
|
Afiliación : |
GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; DRAGOS SCARLET, Division of Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;; MARIA MELCHERT, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; REINHARD ERT, Vetcore Facility, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; CHRISTINE AURICH, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. |
|
Título : |
Epigenetic changes in equine embryos after short-term storage at different temperaturas. |
|
Fecha de publicación : |
2021 |
|
Fuente / Imprenta : |
Animals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325 |
|
DOI : |
10.3390/ani11051325 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria. |
|
Contenido : |
Simple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim
of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development,
maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C);
day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation
(malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There
were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis
(BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and
DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest
some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer. MenosSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and... Presentar Todo |
|
Palabras claves : |
Development; Embryo; Embryo-maternal recognition; Equine; Methylation; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Transport. |
|
Thesagro : |
EQUINOS. |
|
Asunto categoría : |
-- |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16671/1/animals-11-01325.pdf
https://www.mdpi.com/2076-2615/11/5/1325/pdf
|
|
Marc : |
LEADER 04973naa a2200301 a 4500
001 1062116
005 2022-09-02
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3390/ani11051325$2DOI
100 1 $aGASTAL, G.D.A.
245 $aEpigenetic changes in equine embryos after short-term storage at different temperaturas.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria.
520 $aSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer. Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C); day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation (malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis (BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer.
650 $aEQUINOS
653 $aDevelopment
653 $aEmbryo
653 $aEmbryo-maternal recognition
653 $aEquine
653 $aMethylation
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aTransport
700 1 $aSCARLET D.
700 1 $aMELCHERT M.
700 1 $aERTL R.
700 1 $aAURICH, C.
773 $tAnimals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
